Immunofluorescence [IF] Staining (3-plex:DAPI+3 targets)
$120/slide (If antibody is provided by iHisto, an additional cost of $15/antibody)
Click the image to view whole slide scanning
IL2 antibody labeled green, Calbindin antibody labeled red, and IL10 antibody labeled pink
3plex Immunofluorescence (IF) staining, which involves the concurrent detection of three specific target antigens, is a frequently used technique in biological research and clinical diagnostics. It utilizes fluorescent-labeled antibodies tailored for each target antigen, enabling direct visualization of all three simultaneously. Despite its established utility, achieving successful 3plex staining demands meticulous consideration of multiple factors and the implementation of various optimization steps.
Reagent:
In-house
Fluorophore options(choose three):
Blue (375)
Cyan (435)
Green (490)
Orange (545)
Red (590)
Far-red (650)
Infra-red (740)