TSA-based Immunofluorescence [IF] Staining ( stain with the same host antibody)
IF staining fee + $10/antibody
Click the image to view whole slide scanning
![TSA-based Immunofluorescence [IF] Staining ( stain with the same host antibody)](https://static.wixstatic.com/media/0a6309_ddcff0385e79406eb73c160124b14f95~mv2.jpg/v1/fill/w_978,h_589,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/Image-empty-state.jpg)
Figure: Three Rabbit antibodies
Tyramide-based fluorescent mIHC involves HRP-catalyzed deposition of fluorophore-conjugated tyramide molecules at the site of the antigen or in its immediate vicinity. This can markedly enhance the level of signal amplification. Moreover, tyramide deposition is mediated through its covalent binding to tyrosine residues on the antigen and its neighboring proteins. The permanent nature of this binding allows for heat-mediated removal of primary/secondary antibody pairs, while preserving the fluorescence signal associated with the antigen. This facilitates the sequential use of multiple primary antibodies of the same host species or isotype without the concern for crosstalk, thereby greatly enabling the potential to multiplex.
Fluorophore options(choose three):
DAPI (Nuclear)
Alexa Fluor 488
Alexa Fluor 555
Alexa Fluor 594
Alexa Fluor 647
Alexa Fluor 750
Schematic representation of the fluorescent mIHC workflow:
1. Deparaffinization/Rehydration:
2. Antigen Retrieval
3. Staining
4. Scanning
More details please see the link below:
