The Alcian Blue - PAS Stain Kit is intended for use in the simultaneous histological visualization of sulfated and carboxylated acid mucopolysaccharides, sulfated and carboxylated sialomucins and neutral mucins.
Alcian Blue, a copper phthalocyanine dye, binds acid mucosubstances. When used in a pH 2.5 acid solution Alcian Blue stains sulfated and carboxylated acid mucosubstances. Neutral mucins are stained with a standard Periodic Acid Schiff (PAS) reaction. Neutral mucins are oxidized by Periodic Acid forming free aldehydes capable of binding Schiff’s Solution resulting in a magenta end product. Hematoxylin is provided as a nuclear counterstain.
Alcian Blue - PAS Stain Kit
- Acidic Sulfated Mucosubstances: Blue
- Hyaluronic Acid: Blue
- Sialomucins: Blue
- Neutral Mucins: Magenta
- Mixtures of Acidic and Neutral Mucins: Blue - Mauve depending on dominant entity