GRAM Micro-Organisms stain

$12/slide

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GRAM Micro-Organisms stain

Gram Stain Kit (Microorganism Stain) is intended for the
demonstration and differentiation of Gram-positive and Gram-negative bacteria

Procedure:
1. Deparaffinize sections and hydrate in distilled water.
2. Apply adequate Gentian Violet Solution to completely cover
tissue section and incubate for 1 minute.
3. Rinse slide in distilled water to remove excess stain.
4. Apply adequate Lugol’s Iodine Solution to completely cover
tissue section and incubate for 1 minute.
5. Rinse slide in running tap water to remove excess iodine.
6. Place slide in Gram’s Decolorizer until color no longer bleeds off
section.
7. Rinse slide quickly in distilled water.
8. Apply adequate Carbol Fuchsin to completely cover tissue
section and incubate for 1-2 minutes.
9. Rinse slide quickly in distilled water to remove excess stain.
10. Apply Tartrazine Solution and incubate for 15 seconds.
11. Rinse slide one time in absolute alcohol.
12. Dehydrate slide quickly in three changes of absolute alcohol.
13. Clear in two changes of xylene or xylene substitute and mount in
synthetic resin

Result:
Gram Positive Bacteria: Blue
Gram Negative Bacteria: Red
Other Tissue: Yellow
Nuclei: Red

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