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Nissl body, also known as Nissl substance, is a large granular body located in cytoplasm of neurons. The Nissl body major contains ribosome and rough endoplasmic reticulum, is the site of protein synthesis. Distribution of Nissl body is a marker demonstrating neuron injury or not. This staining is commonly used for identifying the basic neuronal structure. This staining method is based on toluidine blue staining.
Nissl bodies are shown in deep blue, nucleus are light blue and background is colorless.