Periodic Acid Schiff (PAS) stain
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Description: This method is used for detection of glycogen in tissues such as liver, cardiac and skeletal muscle on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The glycogen, mucin, and fungi will be stained purple and the nuclei will be stained blue.
Fixation: 10% formalin.
Section: paraffin sections at 5 um.
1) Deparaffinize and hydrate to water.
2) Oxidize in 0.5% periodic acid solution for 5 minutes.
3) Rinse in distilled water.
4) Place in Schiff reagent for 15 minutes (Sections become light pink color during this step).
5) Wash in lukewarm tap water for 5 minutes (Immediately sections turn dark pink color).
6) Counterstain in Mayer's hematoxylin for 1 minute.
7) Wash in tap water for 5 minutes.
8) Dehydrate and coverslip using a synthetic mounting medium.
Glycogen, mucin and some basement membranes: red/purple