Safranin O / Fast green stain
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This method is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed, paraffin-embedded tissue sections, and may be used for frozen sections as well. The cartilage and mucin will be stained orange to red, and the nuclei will be stained black. The background is stained bluish green.
Fixation: Formalin fixed, paraffin embedded sections.
Cytoplasm: bluish green
Cartilage, mucin, mast cell granules: orange to red