Verhoeff Van Gieson / EVG Stain
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This method is used for identifying elastic fibers in tissues such as skin, aorta, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The elastic fibers will be stained blue-black and background will be stained yellow.
Fixation: 10% formalin.
Section: paraffin sections at 5 um.
Elastic fibers: black to blue-black
Nuclei: blue to black
Other tissue elements ---------- yellow