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Immunofluorescence [IF] Staining (6-plex:DAPI+6 targets)
Manual : $240/slide and Leica Bond: $300 (If antibody is provided by iHisto, an additional cost of $15/antibody)
![Immunofluorescence [IF] Staining (6-plex:DAPI+6 targets)](https://static.wixstatic.com/media/0a6309_5104ed36d5aa4a35b73ac935f1c6203d~mv2.png/v1/fill/w_978,h_589,al_c,q_90,usm_0.66_1.00_0.01,enc_avif,quality_auto/Image-empty-state.png)
Gr-1~cyan, CD4~green, CD8~orange, CD45R(b220)~red, F4/80~far red, CD169~infra-red
6plex Immunofluorescence (IF) staining, which involves the concurrent detection of three specific target antigens, is a frequently used technique in biological research and clinical diagnostics. It utilizes fluorescent-labeled antibodies tailored for each target antigen, enabling direct visualization of all three simultaneously. Despite its established utility, achieving successful 6plex staining demands meticulous consideration of multiple factors and the implementation of various optimization steps.
Reagent:
In-house
Fluorophore options(choose four):
Blue (375)
Cyan (435)
Green (490)
Orange (545)
Red (590)
Far-red (650)
Infra-red (740)
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