i1005

Bone decalcification

$11/sample

i1001

Tissue trimming, processing and embedding (FFPE/OCT)

(Leica ASP6025 - Automated Vacuum Tissue Processor)

$11/sample

i1004

FFPE sectioning

with FisherBrand Superfrost plus slide (HistoCore AUTOCUT)

$6 / 2 sections / slide, additional $3 / section

i1003

OCT sectioning

with FisherBrand Superfrost plus slide (Epredia™ CryoStar™ NX50)

$8 / 2 sections / slide, additional $4 / section

i1006

H&E staining

(Leica ST5020-CV5030 Stainer)

$5.5/slide

i1007-SP1

Special staining (Group1)

Alcian blue, Alcian blue+PAS, Masson's Trichrome, Cresyl Echt Violet (Nissl's ), Oil red O, PAS, Safranin O/Fast green, Toluidine blue, Mucicarmine (Modified Southgate's), Periodic Acid Schiff (PAS) Diastase, Picro-Sirius Red (Leica ST5020-CV5030 Stainer)

$11/slide

i1007-SP2

Special staining (Group2)

Gram Micro-Organisms, Luxol Fast Blue, Iron Stain Kit (Prussian Blue Stain), Verhoeff Van Gieson/EVG stain, Colloidal Iron Stain (Mucin Stain), Copper Stain, Grocott Methenamine Silver (GMS) Stain

$16.5/slide

i1007-SP3

Special staining (Group3)

Movat Pentachrome stain, Fontana-Masson Stain (Melanin Stain), Trap stain

$33/slide

i1007-SP4

Special staining (Group4)

Golgi stain (include 30 layer Z-stack scanning image)

$220/sample

i1008

TUNEL staining (Manual Staining)

Fluorescence

$60/slide

i1009-IHC

Immunohistochemistry (IHC) staining (Manual Staining or Leica Bond Rx)

Hematoxylin+1 target

Manual : $40/slide and Leica Bond: $50 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1009-IHC-D

2plex Immunohistochemistry (IHC) staining (Manual Staining or Leica Bond Rx)

Hematoxylin+2 targets

Manual : $80/slide and Leica Bond: $100 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-SIF

Single Immunofluorescence [IF] staining (Manual Staining or Leica bond Rx)

DAPI+1 target

Manual : $40/slide and Leica Bond: $50 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-2IF

2-plex Immunofluorescence [2IF] staining (Manual Staining or Leica bond Rx)

DAPI+2 targets

Manual : $80/slide and Leica Bond: $100 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-3IF

3-plex Immunofluorescence [3IF] staining (Manual Staining or Leica bond Rx)

DAPI+3 targets

Manual : $120/slide and Leica Bond: $150 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-4IF

4-plex Immunofluorescence [4IF] staining (Manual Staining or Leica bond Rx)

DAPI+4 targets

Manual : $160/slide and Leica Bond: $200 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-5IF

5-plex Immunofluorescence [5IF] staining (Manual Staining or Leica bond Rx)

DAPI+5 targets

Manual : $200/slide and Leica Bond: $250 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1011-6IF

6-plex Immunofluorescence [6IF] staining (Manual Staining or Leica bond Rx)

DAPI+6 targets

Manual : $240/slide and Leica Bond: $300 (If antibody is provided by iHisto, an additional cost of $15/antibody)

i1010-HCR-C

CISH (HCR kit)

On Leica Bond RX staining, including Pos/Neg probes, reagents and whole slide imaging but excluding target probes, the new protocol must first undergo a verification plan.

$400/slide

i1010-ACD-C

CISH (ACD kit)

On Leica Bond RX staining, including Pos/Neg probes, reagents and whole slide imaging but excluding target probes, the new protocol must first undergo a verification plan.

$650/slide

i1010-ACD-F

FISH (ACD kit)

On Leica Bond RX staining, Up to DAPI+3 colors (520,570 and 650). Including Pos/Neg probes, reagents, and whole slide imaging but excluding target probes, the new protocol must first undergo a verification plan.

$700/slide

i1010-HCR-CV

CISH Validation plan (HCR kit)

On Leica Bond RX staining, including Pos/Neg probes, reagents and whole slide imaging but excluding target probes. Client need to provide Target probes and a positive sample.

$1600

i1010-ACD-CV

CISH Validation plan (ACD kit)

On Leica Bond RX staining, including pos/Neg probes, reagents and whole slide imaging but excluding target probes. Client need to provide Target probes and a positive sample.

$2600

i1010-ACD-FV

FISH Validation plan (ACD kit)

On Leica Bond RX staining, including pos/Neg probes, reagents and whole slide imaging but excluding target probes. Client need to provide Target probes and a positive sample.

Single: $3000 ; 2-plex: $4500 ; 3-plex: $6000

i1012-6IF-cp

Customized panel (TSA based)

TSA based (up to 6 plex)

Inquire

i3000-SP-S

Spatial Transcriptomic Slide Preparation (suitable for GeoMx and Visium)

Spatial Transcriptomic unstained slides X4, H&E stained slide with Whole slide Image X1 (Fisher Superfrost slides with Nuclease free)

$150

i3001-SP-IPCP

Immune Profiling Core Panel

Akoya phenocycler fusion based: CD3e, CD4, CD8, CD11c, CD14, CD20, CD44, CD45, CD45RO, CD56, CD68, HLA-A, HLA-DR, Ki67, Pan-Cytokeratin

$2,500/slide

i3001-SP-LPM

Lymphocyte Profiling Module panel

Akoya phenocycler fusion based: T-bet, FoxP3, CD39, CD21, TOX, Granzyme B, CD107a, CD79a, TCF-1, CD38

$2,170/slide

i3001-SP-TAM

Tissue Architecture Module panel

Akoya phenocycler fusion based: E-cadherin, Podoplanin, Collagen IV, b-Catenin1, Vimentin, eta-actin, Caveolin, SMA, CD34, CD31

$2,170/slide

i3001-SP-IAPM

Immune Activation & Proliferation Module panel

Akoya phenocycler fusion based: PD-1, PD-L1, ICOS, PCNA, LAG3, IDO1, CD40, VISTA, HLA-E, IFNG

$2,170/slide

i3001-SP-FFMP

FFPE Mouse Panel

Akoya phenocycler fusion based: CD45, CD44, CD3e, CD20, CD68, CD11c, CD31, CD56, CD8, CD45R/B220, CD4, F4/80, Ly6g, Iba1/AIF1, CD206, iNOS, S100A9, MPO, Vimentin, COL1A1, Caveolin-1, αSMA, β-Catenin-1, Pan-Cytokeratin, FoxP3, TOX2, Ki67, PCNA, PDL1, CD36, CD86, COX2, TER119

Inquire

i3001-SP-CP

Customized panel (Akoya based)

Akoya phenocycler fusion based (up to 60 plex)

Inquire

i1015-BF_B

Bright-Field whole slide scanning (Regular size, Basic resolution:41X)

Regular size 1" X 3" , optical magnification 41X, 0.242 um\pixel

$11

i1015-BF2_B

Bright-Field whole slide scanning (Large size, Basic resolution:41X)

Large size 2" X 3" , optical magnification 41X, 0.242 um\pixel

$22

i1015-BF_A

Bright-Field whole slide scanning (Regular size Basic resolution:82X)

Regular size 1" X 3" , optical magnification 82X, 0.121 um\pixel

$33

i1015-BF2_A

Bright-Field whole slide scanning (Large size Basic resolution:82X)

Regular size 2" X 3" , optical magnification 82X, 0.121 um\pixel

$66

i1016-IF_B

Fluorescence whole slide scanning (Basic resolution:50X)

optical magnification 50X, 0.2 um\pixel, DAPI included)

$27.5/antibody (DAPI is free)

i9001-WSI_Q

Whole slide image quantification (HALO)

via Halo software

$20/parameter

i1028

Board-certified veterinary pathologist review and report

Report included

Regular: $80/slide

iPosSP

Positive control for special stain

$10

iPosIHC/IF

Positive control for IHC/IF

$20

i1024

Antibody optimization

pH6 and pH9 retrieval buffer→5 difference Ab concentration plus 1 negative control (2 repeats), total 20 slides

Inquire

i1027-5

Urgent fee (result ready within 5 days)

Total amount X 1.5

i1027-N

Urgent fee (result ready Next day)

Total amount X 2

Shipping & Handling fee (per quote)

Waiver for order over $500

$50

i50box

50 pcs slide box

Providing $10 credit on next quote when returning the slide box

$10

i100box

100 pcs slide box

Providing $15 credit on next quote when returning the slide box

$15

i3002-SP-CJ

Antibody conjugate service for Akoya PhenoCycler System

Antibody conjugate service, sufficient for staining 25 slides. (Not include antibody)

$1,500