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FFPE sectioning (with Fisherbrands superfrost plus slide)

FFPE sectioning (with Fisherbrands superfrost plus slide)


FFPE processing involves fixing the tissue in formalin to preserve its cellular details and embedding it in paraffin wax. The embedded tissue is then sectioned thinly using a microtome and mounted on slides for staining. This method is widely used for diagnostic purposes, including cancer diagnosis. 


  • Excellent Morphology: FFPE preserves cellular and tissue architecture exceptionally well, facilitating detailed histopathological analysis.

  • Long-term Storage: Tissue samples can be stored for extended periods without significant degradation, making them valuable for retrospective studies.

  • Versatility: Compatible with a wide range of staining techniques, including immunohistochemistry (IHC) after antigen retrieval procedures.


  • Time-Consuming: The process is lengthy, requiring days to complete due to the fixation, dehydration, clearing, and embedding steps.

  • Chemical Alteration: Formalin fixation can mask antigens and affect nucleic acids, potentially complicating molecular analyses.

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