In Situ Hybridization Service (ISH)
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HCR/CISH mouse Ppib gene
In Situ Hybridization Service for RNA and DNA Detection
iHisto provides in situ hybridization service for RNA and DNA detection in FFPE and frozen tissue sections. We support RNA ISH, DNA ISH, CISH, FISH, and RNAscope® workflows using optimized probe-based detection and Leica BOND RX automation. Our team helps researchers localize transcripts, gene fusions, viral targets, and other nucleic acid markers while preserving tissue morphology and spatial context in intact tissue sections.
Our In Situ Hybridization Capabilities
Our in situ hybridization service supports a range of assay formats for RNA and DNA detection in FFPE and frozen tissue. Depending on project needs, we offer chromogenic and fluorescent workflows with manual optimization or Leica BOND RX automation to support sensitivity, reproducibility, and efficient turnaround.
In Situ Hybridization Technologies Supported
RNA ISH: Detection of mRNA, lncRNA, non-coding RNA, and viral RNA targets in tissue sections.
DNA ISH: Visualization of gene fusions, copy number variation (CNV), repeat sequences, and other genomic targets.
RNAscope® and HCR: Support for highly sensitive RNA detection workflows using RNAscope® (ACD) and Hybridization Chain Reaction (HCR) technologies.
Custom Probe Strategies: Flexible support for antisense oligonucleotide and locked nucleic acid (LNA) probe designs tailored to specific research targets.
Our In Situ Hybridization Workflow
Our in situ hybridization service follows a streamlined workflow designed to support assay feasibility, consistent tissue processing, and reliable result delivery.
Project Consultation: Review of target, tissue type, species, and study goals
Assay Planning: Selection of probe strategy, detection method, and workflow conditions
Hybridization and Detection: Execution of chromogenic or fluorescent ISH workflows with manual optimization or Leica BOND RX automation
Slide Review and Imaging: Evaluation of stained slides with optional whole slide imaging and pathology review
Result Delivery: Delivery of slides, digital images, and project outputs based on study scope
Common Applications of Our In Situ Hybridization Service
Our in situ hybridization service supports a wide range of research applications requiring spatial localization of RNA or DNA targets within intact tissue architecture. Common use cases include transcript mapping, infectious disease research, genomic analysis, and cell-type identification.
Gene Expression Profiling: Spatial mapping of cytokines, oncogenes, biomarkers, and low-abundance transcripts within tissue sections.
Infectious Disease Research: Detection of viral RNA or DNA targets, including pathogens such as SARS-CoV-2, HPV, CMV, and microbial 16S/18S rRNA signals.
Genomic Analysis: Visualization of chromosomal translocations, gene fusions, copy number variation, and other DNA-based alterations.
Cell-Type and Spatial Biology Studies: Identification of specific cell populations through unique RNA transcript patterns and tissue localization.
Why Choose iHisto for In Situ Hybridization Service
iHisto combines automated ISH platforms, flexible detection options, and digital pathology support to help researchers generate high-quality RNA and DNA localization data in tissue sections with strong reproducibility and efficient turnaround.
Leica BOND RX Automation: Integrated automated workflows for consistent hybridization, detection, and reduced assay variability.
Multiple Detection Modes: Support for CISH for brightfield evaluation, FISH for fluorescent or multiplex analysis, and TSA-amplified ISH for low-abundance targets.
Optimized Assay Performance: Assay conditions are optimized based on tissue type, target abundance, and probe strategy to improve signal quality and reproducibility.
Digital Pathology Delivery: High-resolution whole slide imaging and pathologist-reviewed results are available to support efficient review, data sharing, and project collaboration.
Frequently Asked Questions About Our In Situ Hybridization Service
Q: What sample types do you accept for in situ hybridization service?
A: We support both FFPE and frozen tissue sections for a range of RNA and DNA ISH workflows. Project suitability may depend on tissue quality, fixation, target biology, and assay design.
Q: Do you offer RNAscope® service?
A: Yes. We support RNAscope®-based RNA in situ hybridization workflows for sensitive transcript detection in tissue sections, including automated workflows on Leica BOND RX depending on project requirements.
Q: Can you combine in situ hybridization with IHC or immunofluorescence?
A: Yes. We can support dual ISH-IHC or ISH-IF workflows when compatible with the tissue type, target biology, and study goals.
Q: What deliverables are included with your ISH service?
A: Deliverables may include stained slides, high-resolution whole slide imaging, and optional pathologist-reviewed results depending on the scope of the project.
Q: How do I start an in situ hybridization project with iHisto?
A: Contact our team with your target, species, tissue type, sample format, and project goals. We can help evaluate feasibility and recommend an appropriate ISH workflow.
Request a Quote for In Situ Hybridization Service
At iHisto, we support in situ hybridization projects for RNA and DNA detection in FFPE and frozen tissue sections. Whether you need RNA ISH, DNA ISH, CISH, FISH, or RNAscope®-based workflows, our team can evaluate feasibility and recommend an assay strategy aligned with your research goals.
Contact us today to discuss your target, tissue type, and study goals, and to request a quote for our in situ hybridization service.