Akoya PhenoCycler-Fusion Spatial Biology Services
10–60-Plex Spatial Proteomics for FFPE
Accelerate your research with iHisto’s Akoya-certified PhenoCycler-Fusion spatial biology services. We provide high-plex protein profiling from 10 to 60 markers for FFPE tissue, with staining, imaging, spatial analysis, and expert pathology support available for human and mouse studies.
End-to-End Akoya PhenoCycler-Fusion CRO Services
As an Akoya PhenoCycler service provider, iHisto delivers end-to-end spatial proteomics support from panel selection and tissue preparation through staining, imaging, quality control, spatial analysis, and pathologist review. Our PhenoCycler-Fusion workflows support high-plex protein profiling in FFPE and frozen tissue for immuno-oncology, neuroscience, biomarker discovery, and translational research.
Akoya PhenoCycler-Fusion® System
Why Researchers Choose iHisto for Akoya PhenoCycler Services
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Akoya PhenoCycler-Certified CRO Service Provider
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High-throughput PhenoCycler-Fusion spatial protein profiling
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Human and mouse panels, including custom panels up to 60-plex
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Same-day sample pickup available in the Greater Boston area
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Secure digital image delivery with annotations and web-based viewing
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Optional board-certified pathologist review
Akoya PhenoCycler-Fusion Spatial Biology Workflow
Step 1 – Select Your Panel & Request a Quote
Choose a ready-to-use PhenoCode Discovery Panel or build a custom panel of up to 60 markers. Submit your tissue type (FFPE or frozen), species, and study requirements for a project-specific quote.
Step 2 – Ship or Schedule Sample Pickup
Ship your samples to iHisto or schedule same-day local pickup in the Greater Boston area. Our team will review sample quality and confirm study readiness before processing.
Step 3 – Staining, Imaging & Quality Control
Samples are processed using the Akoya PhenoCycler-Fusion workflow with high-plex staining, imaging, and quality control to generate high-resolution spatial protein data.
Step 4 – Receive Images, Data & Spatial Analysis
Access your high-resolution images and analysis-ready data securely through HistoCloud. Optional HALO® spatial analysis and board-certified pathologist review are available based on study needs.
Applications of Akoya PhenoCycler Spatial Proteomics
Immuno-Oncology
Characterize the tumor microenvironment, immune cell infiltration, cell phenotypes, and spatial interactions at single-cell resolution.
Neuroscience & Neurobiology
Profile neuronal populations, glial cells, neuroinflammation, neurodegeneration, and spatial organization within brain tissue.
Biomarker Discovery
Identify and spatially validate protein biomarkers, therapeutic targets, and cellular phenotypes using high-plex spatial protein profiling.
Autoimmune & Inflammatory Disease
Analyze immune cell populations, tissue architecture, inflammatory pathways, and cell-to-cell interactions within diseased tissue.
Translational Research
Bridge preclinical and clinical studies with spatially resolved protein data from human and mouse tissue.
Frequently Asked Questions (FAQ)
Q: What tissue sample types are accepted for Akoya PhenoCycler spatial analysis?
A: We accept FFPE blocks, FFPE tissue sections, frozen tissue sections, and tissue microarrays (TMAs). Human and mouse samples are routinely supported, with other species evaluated based on project feasibility.
Q: What is the turnaround time for Akoya PhenoCycler services?
A: Standard turnaround time is generally 10 business days after the project scope, panel configuration, tissue requirements, sample readiness, and data deliverables are confirmed. Custom panel development, antibody validation or optimization, large cohorts, advanced spatial analysis, and pathologist review may require additional time. Rush scheduling is available following feasibility review.
Q: Do you provide board-certified pathologist review?
A: Yes. Optional review by board-certified MD and DVM pathologists is available for image interpretation, tissue assessment, biomarker evaluation, and study-specific pathology support.
Q: Can I customize an Akoya PhenoCycler antibody panel up to 60-plex?
A: Yes. iHisto supports custom PhenoCycler panels with 10–60 markers. Researchers can select biomarkers based on study objectives, and additional antibodies can be evaluated for validation and integration into the panel.
Q: What is the difference between PhenoCode Discovery Panels and custom PhenoCycler panels?
A: PhenoCode Discovery Panels provide pre-configured marker sets for applications such as immuno-oncology and neurobiology. Custom PhenoCycler panels allow researchers to select markers based on specific study objectives, tissue types, and biological questions.
Q: What data and analysis are available after PhenoCycler imaging?
A: iHisto can provide high-resolution multiplex images, analysis-ready data, and optional spatial analysis including cell phenotyping, nearest-neighbor analysis, proximity analysis, infiltration analysis, density heatmaps, and pathologist review.