Expert Immunohistochemistry (IHC) & IF Staining Services

Introduction

At iHisto, we provide reliable, high-quality immunohistochemistry (IHC) services combined with optimized IHC staining for a wide range of research and preclinical studies. Our specialized immunohistochemistry service is designed to detect specific antigens in tissue sections using validated antibody protocols and advanced staining techniques. Whether you are studying disease progression, therapeutic response, or protein expression, our IHC stain service helps you generate publication-ready results with clarity and speed.

What is Immunohistochemistry (IHC)?

Immunohistochemistry (IHC) is a powerful technique that uses antibodies to detect specific proteins within preserved tissue sections. This method enables researchers to visualize the spatial distribution and intensity of target antigens, supporting both qualitative and semi-quantitative analysis.

IHC staining is widely used in oncology, neuroscience, immunology, and drug development. At iHisto, our immunohistochemistry services are tailored to your study needs—whether you're working with mouse, rat, or other preclinical tissue models. We support both chromogenic and immunofluorescence (IF) services.

Our IHC Staining Capabilities

We offer a full suite of IHC staining services, including:

• Single-marker IHC staining for targeted protein detection.

• Multiplex IHC (dual or triple stains) for complex co-localization.

• Chromogenic detection (DAB or AP) and Immunofluorescence service options.

• Antigen retrieval (heat-induced or enzymatic) for optimized epitope exposure.

• Frozen and FFPE tissue compatibility.

• Custom primary antibody optimization and validation.

• High-resolution slide scanning and digital image delivery.

Our team utilizes automated staining platforms to ensure consistency, high throughput, and reproducibility across every IHC stain service project.

Our Optimized IHC Workflow

iHisto’s IHC staining services are supported by industry-leading technology:

• Leica BOND RX – Automated, high-throughput platforms for precision staining.

• Leica CV5030 – Automated glass coverslipper for consistent, high-quality slide finishing.

• Antibody Optimization – Comprehensive titration and retrieval buffer testing.

• Validated Controls – Positive and negative controls included for every batch to ensure immunohistochemistry service accuracy.

• Digital Slide Scanning – High-resolution output for review, annotation, or AI analysis.

Step-by-Step IHC Staining Process

1. Antigen Retrieval – Epitopes are exposed via heat- or enzyme-induced retrieval.

2. Blocking – Reduces non-specific binding and endogenous enzyme activity.

3. Primary Antibody Incubation – Tissue is incubated with a validated antibody specific to the target.

4. Secondary Detection – HRP- or AP-linked secondary antibodies bind the primary target.

5. Chromogenic/Fluorescent Reaction – Visualization of the antigen-antibody complex.

6. Counterstaining and Mounting – Slides are dehydrated and coverslipped for permanent storage.

Applications of IHC Staining

Our IHC services are essential for spatially mapping protein expression in:

• Oncology – Tumor subtype characterization and immune markers (CD3, CD8, PD-L1).

• Neuroscience – Glial and neuronal markers (GFAP, NeuN).

• Toxicology – Tissue-specific damage and biomarker response.

• Inflammation – Cytokines and macrophage profiling.

• Biomarker Validation – Supporting preclinical therapeutic studies.

Why Choose iHisto for IHC Services?

• Fast Turnaround: Most IHC stain service projects are completed within 5–7 business days.

• High Reproducibility: Automated systems minimize variation and human error.

• Experienced Team: Deep expertise in histopathology and immunohistochemistry service development.

• Digital Pathology: Integrated whole-slide scanning and cloud access.

Supported Sample Types and Species

To ensure the best results for your immunohistochemistry service, we accept a variety of formats:

• Fresh Tissues: Provided in fixatives such as formalin.

• FFPE Samples: Formalin-fixed paraffin-embedded blocks or unstained slides.

• Frozen Samples: OCT-embedded blocks or pre-cut frozen slides.

You may submit your own primary antibodies or request our assistance in sourcing validated clones for your specific target markers. Our team ensures that every IHC stain service includes optimized dilution, retrieval buffers, and stringent controls.

How to Get Started

Ready to submit your samples? Fill out our online quote request form or contact our team for guidance on antibody selection and staining protocols. We're here to support your research goals from sample prep to slide analysis.

Related Services

• Tissue Embedding Services: Professional preparation for reliable IHC staining.

• Slide Scanning & Digital Pathology: High-resolution imaging for your immunohistochemistry service results.

• Special Histology Stains: Complementary morphological analysis.

Frequently Asked Questions (FAQs)

Q: What is the typical turnaround time for an IHC stain service? A: Most IHC staining projects are completed within 5–10 business days. Timeline may vary based on sample volume and the complexity of antibody validation.

Q: Can I provide my own primary antibodies for the immunohistochemistry service? A: Yes. We welcome user-supplied antibodies. To ensure the highest quality IHC service, please provide datasheets or previous dilution guidelines when submitting.

Q: Do you support multiplex IHC or immunofluorescence services? A: Yes, we offer dual and triple IHC staining with optimized protocols for clear signal separation. For higher-plex needs, please see our multiplex IF service section.

Q: What species and tissue types are compatible with your IHC services? A: We have extensive experience with FFPE and frozen tissues from humans, mice, rats, NHPs, and other preclinical models.