Luxol Fast Blue Stain

This is for staining of myelin/myelinated axons on formalin-fixed, paraffin-embedded brain and spinal cord tissue sections, as well as frozen sections. The myelin, including phospholipids, will be stained blue to green, and the neurons will be stained violet. This stain is commonly used for identifying the basic neuronal structure in brain or spinal cord tissue.
 
Fixation: 10% formalin.
 
Section: paraffin sections at 5-10 um. Frozen sections at 20-30um.
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Result:
Myelin, including phospholipids: blue
Neuron: pink to violet