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The TRAP Stain is used for the staining of tartrate-resistant acid phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts and the activity of osteoclasts. Alkaline phosphatase and tartrate-resistant acid phosphatase are used as markers for osteoblasts and osteoclasts, respectively.
Formalin fixed, paraffin tissue sections.
Mineralized bone MUST be decalcified using EDTA.
Acid-decalcifiers inhibit enzymatic staining.
Osteoclasts: red violet