6-plex Immunofluorescence (IF) Staining Services
Manual : $276/slide and Leica Bond: $345 (If antibody is provided by iHisto, an additional cost of $17/antibody)
Gr-1~cyan, CD4~green, CD8~orange, CD45R(b220)~red, F4/80~far red, CD169~infra-red
6-plex Immunofluorescence Staining: High-Definition Spatial Biology & Six-Marker Multiplexing
Overview
6-plex immunofluorescence staining allows for the simultaneous visualization of six protein targets plus DAPI in a single tissue section. This advanced multiplex IF service offers exceptional power for spatial biology, deep immune profiling, and high-content biomarker studies, providing a comprehensive view of the tissue landscape.
At iHisto, we deliver reliable 6-plex immunofluorescence service using TSA (Tyramide Signal Amplification), expert panel design, and Leica BOND RX automation. This combination enables precise, high-throughput detection with minimal signal overlap, ensuring your IF stain service project produces high-fidelity, publication-ready data.
How 6-Plex Multiplex IF Staining Works
Our immunofluorescence service utilizes a strategic sequential staining approach. To achieve clear distinction among six different markers, we optimize the spectral properties of each fluorophore.
Technical Excellence: When multiple primary antibodies share a host species (e.g., all Rabbit), iHisto employs TSA amplification. This advanced multiplex IF stain service technique ensures sequential amplification without cross-reactivity, allowing for confident marker distinction and superior sensitivity.
Fluorophore Options at iHisto:
Spectrum Channels: FITC, CYAN, CY3, Texas-Red, CY5, CY7.
Nuclear Landmark: DAPI (Blue).
Our Optimized 6-Plex Workflow
iHisto provides a rigorous laboratory framework to ensure the success of your high-plex immunofluorescence service:
Precision Automation: Full workflow execution via the Leica BOND RX platform for unmatched multiplexing consistency.
Advanced TSA Separation: Specialized protocols to ensure clean separation, even with overlapping primary species.
Broad Sample Support: Fully validated for human, mouse, rat, NHP, and xenograft tissues (FFPE or frozen).
Digital Excellence: Multichannel whole-slide scanning with separated channel exports and merged overlays.
Panel Consultation: Expert fluorophore matching and panel design included in every multiplex IF stain service.
Applications of 6-Plex Multiplex IF Services
This level of multiplexing is ideal for projects requiring deep spatial profiling and co-expression analysis of complex cell populations.
Tumor Microenvironment (TME) Deconvolution: Mapping CD3, CD8, FOXP3, PD-1, PD-L1, and Cytokeratin simultaneously.
Neuroimmune Interfaces: Detailed profiling of GFAP, Iba1, NeuN, CD68, CD45, and Olig2.
Stem Cell Differentiation: Tracking SOX2, Nestin, OCT4, GFAP, Olig2, and Ki-67.
Signal Pathway Visualization: Correlating pSTAT3, pERK, pAKT, NF-κB, Ki-67, and HIF1α.
Why Choose iHisto for 6-Plex IF Staining?
Maximum Biological Context: High-complexity support for up to 6 targets + DAPI on a single slide.
Superior Signal Integrity: Advanced TSA technology for high-contrast, low-background imaging.
AI-Compatible Data: Digital formats optimized for image analysis software and AI-based quantification.
Pathologist Integration: Optional expert review and annotation for complex biomarker discovery.
Research to GLP Standards: A trusted IF stain service provider for both discovery and preclinical programs.
Frequently Asked Questions (FAQs)
Q: Is it possible to perform 6-plex IF on FFPE tissue sections?
A: Yes. Our immunofluorescence service is fully optimized for FFPE and frozen sections, utilizing TSA to ensure high-efficiency signal detection despite fixation-induced masking.
Q: How do you prevent cross-reactivity in such high-plex panels?
A: We use TSA-based sequential detection, which physically isolates each marker's signal and allows for antibody stripping between rounds, ensuring no cross-detection.
Q: Do you offer whole-slide scanning for 6-plex panels?
A: Absolutely. We provide high-resolution digital imaging with the ability to visualize and export each channel independently or as a merged multi-color overlay.
Ready for High-Definition Spatial Biology?
iHisto’s 6-plex immunofluorescence staining delivers unmatched precision for spatial mapping and biomarker analysis. From deep immune profiling to multiplex biomarker discovery, our combination of TSA technology, Leica BOND RX automation, and expert support ensures results you can trust.
📩 Contact us today to request a quote or consultation for your 6-plex multiplex IF service.