Description:
Analyzing protein biomarkers through spatial profiling of individual cells is a well-established technique employed by cancer researchers to gain insights into the intricacies of the tumor microenvironment (TME). While this methodology has primarily been designed for human tissue samples, the extensive utilization of mouse models in the field of immuno-oncology (IO) warrants the adaptation of spatial profiling techniques for their application in mice.
Markers:
Immuno-Phenotyping Markers:
CD45
CD44
CD3e
CD20
CD68
CD11c
CD31
CD56
CD8
CD45R/B220
CD4
Myeloid Markers:
F4/80
Ly6g
Iba1/AIF1
CD206
iNOS
S100A9
MPO
Structural Markers:
Vimentin
COL1A1
Caveolin-1
αSMA
β-Catenin-1
Pan-Cytokeratin
Proliferation and Transcription Markers:
FoxP3
TOX2
Ki67
PCNA
Advanced Markers:
PDL1
CD36
CD86
COX2
TER119
Analysis (Indica Halo):
NEAREST NEIGHBOR ANALYSIS ALGORITHM
PROXIMITY ANALYSIS ALGORITHM
INFILTRATION ANALYSIS ALGORITHM
DENSITY HEATMAP ALGORITHM
Details please see : link