FFPE Tissue Sectioning Service: Precision Paraffin Sectioning for Research and Diagnostics 

At iHisto, we provide expert FFPE tissue sectioning services with high-precision microtomy and standardized protocols — delivering clean, consistent sections for histology, IHC, ISH, and molecular analysis.

🧪 Overview 

FFPE (Formalin-Fixed, Paraffin-Embedded) tissue sectioning is the foundation of most histological and molecular pathology workflows. High-quality sectioning is critical for:

• Routine H&E staining – for morphology and diagnostic screening

• Immunohistochemistry (IHC) and in situ hybridization (ISH) – compatible with antigen and nucleic acid retrieval

• Special stains and board-certified pathologist review

• Biobanking and long-term archival – stable FFPE blocks for retrospective or future analysis

• Molecular pathology workflows – including RNA/DNA-safe processing for NGS, PCR, and digital PCR

• Spatial biology & multiplex profiling – embedding protocols optimized for platforms like 10x Visium, NanoString GeoMx, and ACD RNAscope®

At iHisto, we use calibrated microtomes and validated protocols to ensure optimal thickness, smooth ribboning, and artifact-free sections — mounted on premium Fisherbrand™ Superfrost™ Plus slides to ensure maximum tissue adhesion and compatibility with even the most sensitive downstream applications.

🔧 Our Sectioning Capabilities 

• Standard thickness: 4 µm

• Sectioning of:
  Tissue blocks from rodents, humans, and NHPs
  Biopsy samples, organ sections, or tissue arrays

• Serial sectioning and slide labeling included

• Water bath and hot plate drying for optimal adhesion

🧬 Workflow at iHisto 

1. Tissue Trimming & Orientation – Ensures target region is preserved

2. Microtome Sectioning – Precision Leica rotary microtomes used

3. Water Bath Floating – Ribbon sections expanded and flattened

4. Slide Transfer & Drying – Sections transferred to charged slides and dried

5. Optional QA/QC Imaging – Visual inspection or reference H&E

We handle both freshly processed blocks and archival FFPE tissues, and accommodate routine or high-volume sectioning orders.

🔬 Applications of FFPE Sectioning 

• Histopathological diagnosis

• Research studies involving gene expression or protein localization

• Preclinical GLP studies and CRO projects

• Retrospective studies using archived FFPE tissue

• Companion diagnostic and biomarker discovery pipelines

✅ Why Choose iHisto

• ✅ Clean, wrinkle-free, artifact-free ribbon sections

• ✅ Fast turnaround and batch consistency

• ✅ Compatible with IHC, ISH, and special stains

• ✅ High-throughput capability for large studies

• ✅ Optional staining and digital slide scanning available

• ✅ Human, rodent, and primate tissue expertise

❓ FAQs 

• What is FFPE tissue sectioning?
  It refers to cutting thin sections from paraffin-embedded, formalin-fixed tissue blocks for histological or molecular analysis.

• What thickness do you provide?
  We standardly cut 4–6 µm, but can adjust depending on your protocol (2–10 µm upon request).

• Do you section archived blocks?
  Yes — we accept archived FFPE tissue from biobanks, hospitals, or CROs.

• Can you provide stained slides too?
  Absolutely — we offer H&E, IHC, special stains, and slide digitization upon request.

📩 Request a Quote or Consultation 

Need high-quality FFPE tissue sections for your next study? Partner with iHisto for precision sectioning, fast turnaround, and full downstream support.

👉 Request a Quote or email us at info@ihisto.io