Tissue Processing and Embedding Services: FFPE Preparation with Precision and Care 

At iHisto, we offer expert tissue processing and embedding services to prepare your samples for downstream histological analysis. Whether you're working with biopsies, preclinical animal models, or clinical tissues, our automated workflow ensures consistent, high-quality paraffin blocks — ready for sectioning, staining, and digital pathology.

🧬 Overview 

Tissue processing and embedding is the first and most critical step in histology. Proper fixation, dehydration, clearing, and paraffin embedding preserves cellular detail and morphology — laying the foundation for all staining and imaging workflows.

At iHisto, we support both routine FFPE workflows and custom protocols for various tissue types:

• Human, rodent, primate, xenograft, and biopsy tissues

• Standard and large-format blocks

• Fatty, calcified, or delicate specimens

• Client-supplied cassettes or loose tissue samples

All processing is performed using automated closed-system processors for optimal consistency and tissue preservation.

⚙️ Our Tissue Processing Workflow

1. Sample Receipt & Logging
   Barcode tracking and visual inspection for sample integrity

2. Fixation
   Standard 10% NBF or custom fixatives upon request

3. Automated Dehydration & Clearing
   Using alcohol and xylene or xylene substitutes

4. Paraffin Infiltration
   Vacuum-assisted infiltration using validated reagent cycles

5. Tissue Embedding
   Accurate orientation into paraffin blocks using heated embedding centers

6. Block Labeling & Storage
   Blocks labeled and optionally stored or prepared for sectioning

🧪 Applications of Our Embedding Services 

iHisto provides both formalin-fixed paraffin embedding (FFPE) and OCT-based cryo embedding, supporting a wide range of histological and molecular applications:

• Routine H&E staining – for FFPE or frozen section morphology

• Immunohistochemistry (IHC) and in situ hybridization (ISH) – compatible with paraffin or cryosection workflows

• Special stains and board-certified pathologist review

• Biobanking and long-term archival – FFPE blocks or cryobanked OCT tissue for future reanalysis

• Molecular pathology workflows – RNA/DNA-safe processing for PCR, sequencing, or ISH

• Spatial biology & multiplex assays – embedding optimized for platforms like 10x Visium, NanoString GeoMx, and RNAscope®, across FFPE and fresh-frozen formats

✅ Why Choose iHisto

• ✅ Clean, artifact-free blocks with precise orientation

• ✅ Fully automated processing using top-tier systems (e.g., Leica, Sakura)

• ✅ Compatible with downstream IHC, ISH, and spatial biology

• ✅ Low-volume and high-throughput capacity

• ✅ Optional slide sectioning, staining, and digital scanning services

• ✅ Experienced in both GLP and research-only workflows

❓ FAQs 

• What types of tissue do you process?
  Human, rodent, primate, xenograft, tumor, organ, biopsy, and more.

• Do you offer fixation services?
  Yes. We provide standard NBF fixation or can process pre-fixed tissue.

• Can I send fresh samples?
  Yes. We handle fresh, pre-fixed, or postmortem tissue with cold-chain intake.

• Are samples tracked?
  Yes. All samples are barcoded and tracked throughout the workflow.

📩 Request a Quote or Consultation 

Need reliable, high-quality tissue processing and embedding? iHisto is your trusted partner for FFPE sample preparation — from fresh tissue to paraffin block.

👉 Request a Quote or email us at info@ihisto.io